Nicotiana benthamiana protein, NbPCIP1, interacting with Potato virus X coat protein plays a role as susceptible factor for viral infection

AbstractThe interactions of viral coat protein (CP) and host factors play an important role in viral replication and/or host defense mechanism. In this study, we constructed Nicotiana benthamiana cDNA library to find host factors interacting with Potato virus X (PVX) CP. Using yeast two-hybrid assay, we screened 3.3×106 independent yeast transformants from N. benthamiana cDNA library and identified six positive clones. One positive clone, named PVX CP-interacting protein 1 (NbPCIP1), is a plant-specific protein with homologue in N. tabacum (GenBank accession no. AB04049). We confirmed the PVX CP–NbPCIP1 interaction using yeast-two hybrid assay in yeast, protein–protein binding assay in vitro, and bimolecular fluorescent complementation assay in planta. Quantitative real-time RT-PCR analysis showed that the mRNA level of NbPCIP1 increased in PVX-infected N. benthamiana plants as compared to that of healthy plants. The green fluorescent protein (sGFP)-fused NbPCIP1 (NbPCIP1-sGFP) was localized in ER or ER-associated granular-like structure of cells. When we co-express NbPCIP1-sGFP and red fluorescent protein (RFP)-fused PVX CP (PVX CP-RFP), which were introduced by transiently expressing these proteins in N. benthamiana protoplasts and epidermal cells, however, we observed the co-localization of these proteins in the inclusion body-like complex in areas surrounding nucleus. Transient over-expression and transgene silencing of NbPCIP1 assay analysis indicated that NbPCIP1 plays a critical role in viral replication during PVX infection in host plant

Availability of hairy vetch (Vicia villosa Roth) as leguminous green manure crops for organic rice in reclaimed saline land

Hairy vetch (Vicia villosa) is playing an important role to improve soil physical properties and soil fertility for the supply of crop nutrients and this crop is also used as a representative legumes green manure for the production of organic agricultural food. In this study we evaluated the availability of hairy vetch in reclaimed organic rice cultivation. The response to increasing salinity was analyzed by means of the germination rate and seedling growth of hairy vetch. Results showed that seed germination of hairy vetch decreased insignificantly up to concentration of 0.6% NaCl. However, shoot and root growth of hairy vetch showed significant reduction at salinity concentrations higher than 0.1% NaCl level. In these results we were considered that hairy vetch can be use under concentration of 0.1% salinity as green manure for reclaimed saline agriculture

Tectonics of the Afar triple junction from InSAR and GPS derived strain maps and seismicity

Strain and seismicity show us the mode by which deformation is accommodated in rifting continents. Here we present a combined analysis of InSAR and GPS derived strain maps and seismicity to understand the tectonics of the current Afar triple junction plate boundary zone. Our results show that that the plate spreading motion is accommodated in different ways in the Red Sea Rift after jumping southeastward along the Gulf of Aden Rift. At the Red Sea Rift, extension and shear are coupled with seismicity, occurring both along-rift but also in areas off-rift. In the Gulf of Aden Rift extension and normal faulting occur in the central parts of the rifts while at the rifts tips strike-slip earthquakes are observed. The extensional strains occur over a broad zone encompassing several overlapping rifts. Conversely the strike-slip earthquakes are focused along a narrow EW trending lineament. The pattern suggests that the recent history of magmatic intrusions in the Red Sea Rift still dominates the plate boundary deformation inducing earthquakes even in areas off-rift and with no previous faults mapped. On the other hand, in the Gulf of Aden Rift our strain and seismicity maps are consistent mainly with extensional tectonics occurring over an exceptionally broad zone (over 200 km). We interpret the strike-slip earthquakes observed at the rift tips as the result of shearing at the rifts tips where the extension terminates against continental lithosphere

Damage Proxy Map from Interferometric Synthetic Aperture Radar Coherence

A method, apparatus, and article of manufacture provide the ability to generate a damage proxy map. A master coherence map and a slave coherence map, for an area prior and subsequent to (including) a damage event are obtained. The slave coherence map is registered to the master coherence map. Pixel values of the slave coherence map are modified using histogram matching to provide a first histogram of the master coherence map that exactly matches a second histogram of the slave coherence map. A coherence difference between the slave coherence map and the master coherence map is computed to produce a damage proxy map. The damage proxy map is displayed with the coherence difference displayed in a visually distinguishable manner

Development of Pd Alloy Hydrogen Separation Membranes with Dense/Porous Hybrid Structure for High Hydrogen Perm-Selectivity

For the commercial applications of hydrogen separation membranes, both high hydrogen selectivity and permeability (i.e., perm-selectivity) are required. However, it has been difficult to fabricate thin, dense Pd alloy composite membranes on porous metal support that have a pore-free surface and an open structure at the interface between the Pd alloy films and the metal support in order to obtain the required properties simultaneously. In this study, we fabricated Pd alloy hydrogen separation membranes with dense/porous hybrid structure for high hydrogen perm-selectivity. The hydrogen selectivity of this membrane increased owing to the dense and pore-free microstructure of the membrane surface. The hydrogen permeation flux also was remarkably improved by the formation of an open microstructure with numerous open voids at the interface and by an effective reduction in the membrane thickness as a result of the porous structure formed within the Pd alloy films

Feasible economic strategies to improve screening compliance for colorectal cancer in Korea

Aim: While colorectal cancer (CRC) is an ideal target for population screening, physician and patient attitudes contribute to low levels of screening uptake. This study was carried out to find feasible economic strategies to improve the CRC screening compliance in Korea. Methods: The natural history of a simulated cohort of 50-year-old Korean in the general population was modeled with CRC screening until the age of 80 years. Cases of positive results were worked up with colonoscopy. After polypectomy, colonoscopy was repeated every 3 years. Baseline screening compliance without insurance coverage by the national health insurance (NHI) was assumed to be 30%. If NHI covered the CRC screening or the reimbursement of screening to physicians increased, the compliance was assumed to increase. We evaluated 16 different CRC screening strategies based on Markov model. Results: When the NHI did not cover the screening and compliance was 30%, non-dominated strategies were colonoscopy every 5 years (COL5) and colonoscopy every 3 years (COL3). In all scenarios of various compliance rates with raised coverage of the NHI and increased reimbursement of colonoscopy, COL10, COL5 and COL3 were non-dominated strategies, and COL10 had lower or minimal incremental medical cost and financial burden on the NHI than the strategy of no screening. These results were stable with sensitivity analyses. Conclusion: Economic strategies for promoting screening compliance can be accompanied by expanding insurance coverage by the NHI and by increasing reimbursement for CRC screening to providers. COL10 was a cost-effective and cost saving screening strategy for CRC in Korea. ？ 2005 The WJG Press and Elsevier Inc. All rights reserved.http://www.wjgnet.com/1007-9327/11/1587.aspOAIID:oai:osos.snu.ac.kr:snu2005-01/102/0000052039/4SEQ:4PERF_CD:SNU2005-01EVAL_ITEM_CD:102USER_ID:0000052039ADJUST_YN:YEMP_ID:A077862DEPT_CD:801CITE_RATE:0FILENAME:3_Feasible economic strategies to improve screening compliance for colorectal cancer in Korea.pdfDEPT_NM:의학과EMAIL:[email protected]: